Muscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic T cells and upregulation of regulatory T cells.

Sodium iodide symporter PET and BLI noninvasively reveal mesoangioblast survival in dystrophic mice / Holvoet, Bryan; Quattrocelli, Mattia; Belderbos, Sarah; Pollaris, Lore; Wolfs, Esther; Gheysens, Olivier; Gijsbers, Rik; Vanoirbeek, Jeroen; Verfaillie, Catherine M.; Sampaolesi, Maurilio; Deroose, Christophe M.. - In: STEM CELL REPORTS. - ISSN 2213-6711. - 5:6(2015), pp. 1183-1195. [10.1016/j.stemcr.2015.10.018]

Sodium iodide symporter PET and BLI noninvasively reveal mesoangioblast survival in dystrophic mice

SAMPAOLESI, MAURILIO;
2015

Abstract

Muscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic T cells and upregulation of regulatory T cells.
2015
biochemistry; cell biology; developmental biology; genetics
01 Pubblicazione su rivista::01a Articolo in rivista
Sodium iodide symporter PET and BLI noninvasively reveal mesoangioblast survival in dystrophic mice / Holvoet, Bryan; Quattrocelli, Mattia; Belderbos, Sarah; Pollaris, Lore; Wolfs, Esther; Gheysens, Olivier; Gijsbers, Rik; Vanoirbeek, Jeroen; Verfaillie, Catherine M.; Sampaolesi, Maurilio; Deroose, Christophe M.. - In: STEM CELL REPORTS. - ISSN 2213-6711. - 5:6(2015), pp. 1183-1195. [10.1016/j.stemcr.2015.10.018]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1582029
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